Re: hydrogen peroxide treatment on IHC

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From:Shelley Sheridan <>
To:HistoNet Server <>
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<P>We treat all of our IHC with a Methanol / H2O2 solution that consists of 150 mL Methanol & 30mL of 30%H2O2.  We treat our sections for 30 minutes and then rinse in running tap water for 10 minutes.  This is to help block the endogenous peroxidase and therefore have less background.</P> <P> </P><BR><BR>Shelley K. Sheridan<br>Research Specialist<br>Center for Neurodegenerative Disease Research<br>University of Pennsylvania Medical School<br>Philadelphia, PA 19104<br>Phone:(215)614-0051<p><br><hr size=1><b>Do You Yahoo!?</b><br> <a href="">Yahoo! Photos</a> -- now, 100 FREE prints!
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