Dear Michelle   I take it that you have unfixed cryostat sections If i
were you I would try to fix them in 4% Paraformaldehyde try about 30mins
to start with. Dehydrate ,defat in xylene then section down to water,
stain in cresyl( my recipe for this is a 0.1% soln cresylviolet in
5%acetic acid then pHto 3.5 with sodium acetate) for about 2mins then wash
well in water and differenciate in ascending alcohols,clear and mount.If
differenciation is a problem you can use a weak acid alcohol.Hope this
helps
p.s  the pH of stain is fairly critical  

 Mary Latimer
 School of Psychology
 University of St-Andrews 
 St-Andrews 
 Fife
 tel  01334 463046
 fax  01334 463042


On Tue, 6 Jun 2000 Michelle.Taurino@aventis.com wrote:

> Hello-
> 
> Does anyone have any suggestions/protocol modifications for performing
> cresol etch violet on unfixed rat brain?
> 
> Thanks-
> Michelle Taurino
> Aventis Pharmaceuticals
> Bioimaging and Molecular Histology
> Tel-908-231-3357
> Fax-908-231-3962
> e-mail: Michelle.Taurino@aventis.com
> 
> 
>