Re: Paraffin Embedding Media

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From:Terry J Mattoon <>


I've been a Histo Tech since 1976 and have used a variety of different
embedding mediums.  I have liked Paraplast & Paraplast Plus the best.  It
has the melting point that
works for us (50-55degrees).  We set our water bath at 45 degrees, we put
our rack of slides in an oven at 75 degrees for 30 minutes and we have
three changes of xylene that we start our staining off in.  The first two
are for 5 minutes each and the third one is for 1 minute.  We then have
graduated alcohols to distilled water .  We routinely stain in
Hematoxylin and Eosin  (Richard-Allan) Brand.  It works for me!  Good
results, crisp and clear.  Hope that helps.

Terry Mattoon
VA Medical Center
White River Jct., VT 05001

On Fri, 02 Jun 2000 19:44:43 +0000 (GMT) writes:
> Hi,
> I'm a research assistant at Lawrence Berkeley Lab and we're working 
> on a 
> new protocol using paraffin embedding and I was wondering if you 
> could 
> offer advice on what type to use.  We're doing studies that require 
> sections of 4um as well as 40-80um, so what melting point would be 
> best 
> for paraffin wax?  We were thinking of a 50-55C mp range, but any 
> suggestions?  Also, I've found embedding medias by Fisher which use 
> low 
> molecular polymers along with purified paraffins.  Has anyone used 
> this 
> and any input on its effects on staining procedures?  Thanks for 
> your 
> help.
> Saori Haigo

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