Re: Mowiol.
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From: | diana_papoulias@usgs.gov |
To: | "Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
Add 36 g glycerol to a beaker
add 14.4 g of Mowiol
stir with a spatula to mix then add 36 ml of ddH20, cover with parafilm and
contiue to stir with a stir bar overnight at room temperature
On the following day, add 72 ml of 0.2 M Tris (0.884 g Tris HCl/100 ml
+1.744 g Tris base/100 m; pH 8.5) and heat to 50C for 10 min with
occasional stirring. Then add a stir bar and continue stirring until the
Mowiol dissolves
Once dissovled, centrifuge at 5000 x g for 15 min to clarify
Aliquot into airtight tubes and freeze at -20C
Shelf life at room temperature after thawing is several weeks to months.
(This protocol was originally obtained from the Molecular Cytology Core
Facility, University of Missouri, Columbia, Missouri)
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