I use TH (tyrosine hydroxylase) to label dopamine neurons also. I believe I used an antibody from Boehringer Mannheim (monoclonal), and it works very nicely. I routinely use the Vector Elite ABC kit for immuno, with H2O2/Methanol pretreatment, and microwaving in 10 mM citrate buffer pH 6.0.
Joyce Kotzuk, Univ. of New Mexico pathology deptl;

>>> Shelley Sheridan <sksherid@yahoo.com> 06/07/00 01:20PM >>>

We label Dopaminergic Neurons with a Tyrosine Hydroxolase antibody. 


Shelley K. Sheridan
Research Specialist
Center for Neurodegenerative Disease Research
University of Pennsylvania Medical School
Philadelphia, PA 19104
Phone:(215)614-0051


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