--- Luis Chiriboga <luis.chiriboga@med.nyu.edu> wrote:
> Dear Histonetters:
> I am taking an informal survey to see what HIER
> methods/techniques
> everyone is using.  As with any survey,  the more
> responses the better.
> If anyone has additional questions they would like
> to add please e-mail
> me directly and i will reissue a questionnaire.
> 
> 1) Which heating method do you use for HIER? (i.e..
> Microwave, pressure
> cooker, combination etc...)
steamer
> 2) What is the average "real" heating time for
> antigen retrieval?
> (actual boiling time)
it varies with the antibody, anywhere from 20 to 30
minutes.
> 4) How long is your cooling period?
30 minutes in a fumehood
> 5) Do you perform manual or automated IHC?
manual
> 5) Do you prepare your own retrieval solutions or
> purchase commercial?
commercial 10X concentrates.  I've learned the hard
way that making it up all the time is more time
consuming than is necessary.
> 6) Do you use more than one HIER solution? (please
> list)
Target (Citrate) from Dako
EDTA - Zymed
Glyca- Biogenex
> 7) Which solution do you use the most?
> It's a tie between Target and EDTA

> I will post the stats as soon as I compile all the
> responses.
> Thanks in advance
> 
> 
> 
> --
> Luis Chiriboga Ph.D HT(ASCP)QIHC
> Department of Pathology 4W27
> Bellevue Hospital
> NYU School of Medicine
> First Avenue & 27th Street
> New York, NY 10016
> (212)562-4667
> 
> 
> 
> 


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