Hi,
    1) steamer and waterbath (for Herceptest)
    2) 20 min.
    3) it is not a time it is a temperature (avg. about 10 min) until the
temperature drops to about 40 degrees C
    4) automated
    5) we purchase concentrated retrieval solutions
    6) We use three: Herceptest retrieval solution, and two different pH
solutions
    7) pH 6.5 is the most commonly used for us
Amos Brooks

Luis Chiriboga wrote:

> Dear Histonetters:
> I am taking an informal survey to see what HIER methods/techniques
> everyone is using.  As with any survey,  the more responses the better.
> If anyone has additional questions they would like to add please e-mail
> me directly and i will reissue a questionnaire.
>
> 1) Which heating method do you use for HIER? (i.e.. Microwave, pressure
> cooker, combination etc...)
> 2) What is the average "real" heating time for antigen retrieval?
> (actual boiling time)
> 4) How long is your cooling period?
> 5) Do you perform manual or automated IHC?
> 5) Do you prepare your own retrieval solutions or purchase commercial?
> 6) Do you use more than one HIER solution? (please list)
> 7) Which solution do you use the most?
>
> I will post the stats as soon as I compile all the responses.
> Thanks in advance
>
> --
> Luis Chiriboga Ph.D HT(ASCP)QIHC
> Department of Pathology 4W27
> Bellevue Hospital
> NYU School of Medicine
> First Avenue & 27th Street
> New York, NY 10016
> (212)562-4667