Connie
We also use glycerin gelatine but instead of phenol we use thymol which is
more user friendly. We also use a small amount of bromothymol blue as an
indicator when we are adjusting the pH.
Barry

Connie McManus wrote:

> Pravin,
>
> I don't know what  Vectashield is, but for my IHC slides, I use glycerin
> jelly.  This is water soluble, and works well for me.
>
> Gelatin..................10 g
> DI Water.................60 mL
> Dissolve gelatin with heat
>
> Add:
> Glycerin.................70 mL
> Phenol...................1 mL
>
> Mix well.  To use, heat at 60 degrees C in a beaker of water.
>
> connie m
>
> At 06:02 AM 06/16/2000 -0700, Pravin Patil wrote:
> >I tried a hydrated
> >mount like vectashield, but that washed out the blue
> >stain.  Any Ideas?  I would rather not use any alcohol
> >processing, as it may destroy the GFP signal..
> >
> >
> Connie McManus
> Veterinary Diagnostics Lab
> Utah State University
> Logan, UT
> USA