Pravin,

I don't know what  Vectashield is, but for my IHC slides, I use glycerin
jelly.  This is water soluble, and works well for me.  

Gelatin..................10 g
DI Water.................60 mL
Dissolve gelatin with heat

Add:
Glycerin.................70 mL
Phenol...................1 mL

Mix well.  To use, heat at 60 degrees C in a beaker of water.

connie m

At 06:02 AM 06/16/2000 -0700, Pravin Patil wrote:
>I tried a hydrated
>mount like vectashield, but that washed out the blue
>stain.  Any Ideas?  I would rather not use any alcohol
>processing, as it may destroy the GFP signal..
>
>
Connie McManus
Veterinary Diagnostics Lab
Utah State University
Logan, UT
USA