Re: CD3 and CD79a on decal
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From: | DDittus787@aol.com |
To: | dsitrin@unipathllc.com, histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="US-ASCII" |
If you are using any retreival techniques on B5 fixed tissue you will get
false negativity, after multiple trials on a wide variety of antibodies fixed
this way, I have found that tissue fixed in b5 needs no retrieval at all,
especially for cd markers. I have only experienced false positivity with
steam protocols and by actual testing created my own false positives on neg
tissue.
Dana
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