RE: Wade Fite Modification
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From: | "Kellar, Eric" <kellarec@MSX.UPMC.EDU> |
To: | 'histonet' <histonet@pathology.swmed.edu>, 'David Grehan' <david.grehan@olhsc.ie> |
Reply-To: | |
Content-Type: | text/plain |
Differentiation must be carefully controlled. A faint residual reddish pink
color in the tissues is especially important, and sections that do not show
this (by over differentiation) may be unreliable for the exclusion of
leprosy.
Drury & Wallington, Carleton's Histological Technique, Fourth Edition,
Oxford University Press, 1967.
Eric C. Kellar
> ----------
> From: David Grehan[SMTP:david.grehan@olhsc.ie]
> Sent: Friday, June 16, 2000 9:54 AM
> To: 'histonet'
> Subject: (no subject)
>
> HELLO
> Does anyone know how to remove excess background carbol fuchsin stain
> when doing the Wade Fite modification of the ZN method. I
> differentiate with 10% sulphuric acid but find it difficult to get a
> relatively clean background. I can see the bugs but the back ground is
> still very pink.
>
>
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