RE: Wade Fite Modification

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From:"Kellar, Eric" <kellarec@MSX.UPMC.EDU>
To:'histonet' <histonet@pathology.swmed.edu>, 'David Grehan' <david.grehan@olhsc.ie>
Reply-To:
Content-Type:text/plain

Differentiation must be carefully controlled. A faint residual reddish pink
color in the tissues is especially important, and sections that do not show
this (by over differentiation) may be unreliable for the exclusion of
leprosy. 

Drury & Wallington, Carleton's Histological Technique, Fourth Edition,
Oxford University Press, 1967.


Eric C. Kellar



> ----------
> From: 	David Grehan[SMTP:david.grehan@olhsc.ie]
> Sent: 	Friday, June 16, 2000 9:54 AM
> To: 	'histonet'
> Subject: 	(no subject)
> 
> HELLO
> Does anyone know how to remove excess background carbol fuchsin stain
> when doing the  Wade Fite modification of the ZN method.  I
> differentiate with 10% sulphuric acid but find it difficult to get a
> relatively clean background. I can see the bugs but the back ground is
> still very pink.
> 
> 



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