RE: Paraffin Embedding Media
<< Previous Message | Next Message >>
From: | "Smith, Allen" <asmith@mail.barry.edu> |
To: | "'SLHaigo@lbl.gov'" <SLHaigo@lbl.gov> |
Reply-To: | |
Content-Type: | text/plain; charset="iso-8859-1" |
I have been using "Paraplast" for 35 years, and I am very happy with it.
Paraplast melts at 56 C; Paraplast X-tra melts at 52 C. Paraplast is made
by Sherwood, but Fisher and many other distributors sell it.
Allen A. Smith
School of Graduate Medical Sciences
Barry University
Miami Shores, FL 33161
-----Original Message-----
From: SLHaigo@lbl.gov [mailto:SLHaigo@lbl.gov]
Sent: Friday, June 02, 2000 3:45 PM
To: histonet@pathology.swmed.edu
Subject: Paraffin Embedding Media
Hi,
I'm a research assistant at Lawrence Berkeley Lab and we're working on a
new protocol using paraffin embedding and I was wondering if you could
offer advice on what type to use. We're doing studies that require
sections of 4um as well as 40-80um, so what melting point would be best
for paraffin wax? We were thinking of a 50-55C mp range, but any
suggestions? Also, I've found embedding medias by Fisher which use low
molecular polymers along with purified paraffins. Has anyone used this
and any input on its effects on staining procedures? Thanks for your
help.
Saori Haigo
<< Previous Message | Next Message >>