RE: Paraffin Embedding Media

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From:"Smith, Allen" <>
To:"''" <>
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I have been using "Paraplast" for 35 years, and I am very happy with it.
Paraplast melts at 56 C; Paraplast X-tra melts at 52 C.  Paraplast is made
by Sherwood, but Fisher and many other distributors sell it.
				Allen A. Smith
				School of Graduate Medical Sciences
				Barry University 
				Miami Shores, FL 33161

-----Original Message-----
From: []
Sent: Friday, June 02, 2000 3:45 PM
Subject: Paraffin Embedding Media


I'm a research assistant at Lawrence Berkeley Lab and we're working on a 
new protocol using paraffin embedding and I was wondering if you could 
offer advice on what type to use.  We're doing studies that require 
sections of 4um as well as 40-80um, so what melting point would be best 
for paraffin wax?  We were thinking of a 50-55C mp range, but any 
suggestions?  Also, I've found embedding medias by Fisher which use low 
molecular polymers along with purified paraffins.  Has anyone used this 
and any input on its effects on staining procedures?  Thanks for your 

Saori Haigo

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