Donna,
what type of decal solution is being used and how long are the sections in
the solution? 
	 I have had problems with many false negatives in decal blocks and
have also experienced  non-specific staining with decals.  We use Redecal
from StatLab Medical Products, it contains a 12.% % v/v HCL with 0.26% w/v
Disodium EDTA Dihydrate and have had better immuno results.  We've tried
Nitric Acid solutions and other commercial products, but we have good
success with Redecal.  StatLab's number is 1-800-442-3573.  Hope this helps.

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas 

> -----Original Message-----
> From:	Donna Sitrin [SMTP:dsitrin@unipathllc.com]
> Sent:	Thursday, June 08, 2000 2:15 PM
> To:	histonet@pathology.swmed.edu
> Subject:	CD3 and CD79a on decal
> 
> Okay all you immuno wizards out there--I have a couple of questions.
> 
> Has anybody experienced false positives on CD3, and false negatives on
> CD79a?  If you have researched this problem, please share your knowledge
> and experience.  These particular cases are exposed to decal solution, as
> well as B5 fixative.
> 
> Thanks in advance for some much needed help.
> 
> Donna Sitrin
> dsitrin@unipathllc.com
> Mgr. Tech. Svcs.
>