Tammy,
we use CD-23 from Biocare Medical with their EDTA retrieval solution.  We
steam for 20 minutes and have a 30 minute incubation in the primary, link
and label.  We have obtained great results at 1:50.  Hope this helps.

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas 


> -----Original Message-----
> From:	Barnhart, Tammy [SMTP:tbarnhart@primecare.org]
> Sent:	Thursday, June 08, 2000 12:17 PM
> To:	histonet@pathology.swmed.edu
> Subject:	CD-23
> 
> Looking for some help.....I am trying to get CD-23 (Cell Marque, clone
> 1B12) to stain and am not having very good luck.  I'm using HEIR, 1:50
> dilution with 15 minute detection times.  This protocol ( with a few minor
> adjustments, depending on the antibody)  works well in our lab with other
> antibodies.  Not so good with this one.  Any suggestions??  Jim B, got any
> ideas?  Thanks in advance.
> 
> Tammy Barnhart, BS, HTL(ASCP)
> St. Alexius Medical Center
> Bismarck, ND
> tbarnhart@primecare.org
>