Hi,
I have been using Dako pronase heated for 15 mins at 37C and applied to
sections for 1 minute before pressuring cooking these sections for 2
mins at full pressure. I do this for some antibodies I think require
enhanced antigen retreival e.g CD15, CD1A, Tdt.
There is a definite improvement in staining.
Does anyone else do similar. I would be interested in hearing your
views.