Pronase before HIER

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From:David Grehan <>
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I have been using Dako pronase heated for 15 mins at 37C and applied to
sections for 1 minute before pressuring cooking these sections for 2
mins at full pressure. I do this for some antibodies I think require
enhanced antigen retreival e.g CD15, CD1A, Tdt.
There is a definite improvement in staining.
Does anyone else do similar. I would be interested in hearing your

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