HIER method for CD31
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| From: | Carrie Cartwright <Carrie.Cartwright@Agouron.COM> |
| To: | "histonet@pathology.swmed.edu" <histonet@pathology.swmed.edu>, "'HistoNet@Pathology.swmed.edu'" <HistoNet@pathology.swmed.edu> |
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| Content-Type: | text/plain; charset=us-ascii |
Hi All!
I was wondering if anyone could make a suggestion on what HIER
method I should try. I work with whole retinas. I fix in 10% NBF. I
am using CD31 to look at the vasculature of the retina. I read that
microwave HIER is recommended for CD31, however I haven't found anyone
who does it on whole mounts. Can anyone give me some hints and possibly
suggest a protocol I could try?
Thanks for your time.
Carrie
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