HIER method for CD31

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From:Carrie Cartwright <Carrie.Cartwright@Agouron.COM>
To:"histonet@pathology.swmed.edu" <histonet@pathology.swmed.edu>, "'HistoNet@Pathology.swmed.edu'" <HistoNet@pathology.swmed.edu>
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Hi All!
    I was wondering if anyone could make a suggestion on what HIER
method I should try.  I work with whole retinas.  I fix in 10% NBF.  I
am using CD31 to look at the vasculature of the retina.  I read that
microwave HIER is recommended for CD31, however I haven't found anyone
who does it on whole mounts.  Can anyone give me some hints and possibly
suggest a protocol I could try?
Thanks for your time.

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