H.Pylori immuno staining -Reply

<< Previous Message | Next Message >>
From:Richard Cartun <Rcartun@harthosp.org>
To:Jig1357@aol.com, histonet@pathology.swmed.edu
Content-Type:text/plain; charset=US-ASCII

In my article in Modern Pathology back in 1981 I used a mAb from Bioproducts for Science.  More recently we have been using DAKO's pAb (Code No. B 0471).  This antibody works very well following heat-induced epitope retrieval, although the "Specification Sheet" recommends proteolytic digestion.  We use it at a 1:200 dilution for a 30' incubation or 1:2,000 overnight.  For some reason the antibody is not listed in DAKO's 1999/2000 catalog.

R. Cartun

<< Previous Message | Next Message >>