DAB disposal

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From:Louise Taylor <louiset@mail.saimr.wits.ac.za>
To:histonet <histonet@pathology.swmed.edu>
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Dear histonetters,

Could someone please explain the chemistry behind the potassium
permanganate/sulphuric acid method of DAB disposal?.
Also, what is the purpose of the decolourization step(ascorbic acid) and the
subsequent neutralization. Is there a preferred choice of neutralizer? I
have seen methods that use sodium hydroxide, sodium carbonate, and sodium
bicarbonate - as neutralizer - which is the best?

best regards
louise taylor

Research Laboratory
Department of Anatomical Pathology
South African Institute for Medical Research
South Africa

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