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From:"Michelle M. Sutorik" <>
To:"HistoNet Server" <>
Date:Tue, 27 Jul 1999 15:14:24 -0400
Content-Type:text/plain; charset="us-ascii"

Hello Fellow netters,

I am in need of some help.  I have been attempting to stain for TGFB1 in
several different tissues with some success but am having a great deal of
inconsistant staining within a particular stain.  I need control tissue.  I
have tried Oral squamous cell carcinoma, human colon, as well as an
experimental model that we have.  One is there something i am not
considering that is needed for retrieving growth factors?  I think this may
be a factor also.  TGFB1 expression is differentially expressed so it is
difficult to tie down a control.

Any suggestions are greatly appreciated.
Thanks everyone,

Michelle M. Sutorik
Univ. of MIch.
Dept of Oral Pathology/oncology

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