rapid Oil red O, fixation

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu>
To:histonet@Pathology.swmed.edu
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Date:Wed, 21 Jul 1999 15:39:25 -0600
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Our frozens are cut, air dried briefly, but can be immersed from
croystat right into NBF, and I fix in the 
LIQUID NBF for 1 min, rinse, stain.  If you keep the coplin jar tightly 
sealed with a screw top lid, you may be able to avoid fumes OR take the
section, let it air dry until you can immerse in NBF, inside a fume hood.

Rinse NBF off gently by "swishing" ( a slow waving motion)
slide in tap water, then go to the ORO.  
The short fixation time is no problem, section stays on slide and 
morphology is good, avoids hot formalin fumes. Just use the same fixative
you use for surgical specimens.    

This was our standard fixation for a renal biopsy frozen, and it improved
the quality of section and staining tremendously.  Better than acetone or
95% ethanol.

Gayle Callis



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