mast cell binding avidin
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From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Fri, 23 Jul 1999 09:07:08 -0600 |
Content-Type: | |
What about using a different method to avoid the ABC (avidin kit)
such as a purified primary and a directly labelled (HRP or alk phos)
secondary, and take the avidin/biotin out of the loop entirely.
You might lose a bit of sensitivity, hope I said that correctly!, but
it would remove this mast cell binding to avidin problem. Just a thought.
Gayle Callis
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