formalin fixing already frozen muscle

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From:"Margaret Gondo" <>
Date:Thu, 22 Jul 1999 08:02:40 -0500
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Hi Folks!

Here's the "stupid" question of the day that I hope you all can answer.  I
have some muscle tissue which I had frozen using standard techniques (ie
gum tragacanth, pre-cooled isopentane in liquid nitrogen).  Due to some
questions which have popped up regarding the possibility of infectious
agents being present, I am now being persuaded to formalin fix these same
tissues.  I shrudder to think what these tissues are going to look like
after I'm through thawing, fixing, processing, ect.  Any suggestions as to
how I can do this with the minimalist damage?  Any advice as to what I
should expect my tissue to look like after I'm through?

Thanks a bunch!

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