formalin fixing already frozen muscle
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From: | "Margaret Gondo" <gondom@valentis.com> |
To: | Histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Thu, 22 Jul 1999 08:02:40 -0500 |
Content-Type: | text/plain; charset=us-ascii |
Hi Folks!
Here's the "stupid" question of the day that I hope you all can answer. I
have some muscle tissue which I had frozen using standard techniques (ie
gum tragacanth, pre-cooled isopentane in liquid nitrogen). Due to some
questions which have popped up regarding the possibility of infectious
agents being present, I am now being persuaded to formalin fix these same
tissues. I shrudder to think what these tissues are going to look like
after I'm through thawing, fixing, processing, ect. Any suggestions as to
how I can do this with the minimalist damage? Any advice as to what I
should expect my tissue to look like after I'm through?
Thanks a bunch!
Margaret
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