cracked brain blues

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From:"Andrea Kaj" <>
Date:Thu, 22 Jul 1999 00:04:42 PDT
Content-Type:text/plain; format=flowed

Dear Bruce
It is well known that brain tissue is the main cause of laboratory blues! No 
matter which size, origin and fixative, it certainly can drive you crazy.

I have worked quite a lot with PFA-perfused rat brain, I do not know if my 
method will help you, but you might give it a try.
After perfusion with saline and then 4% PFA, I fix the slice (around 
5mm.)1-4 hours in the fridge (bring cold! PFA to the stables - I transport 
the bucket in ice). I do not use cryoprotection, just wipe off excess liquid 
after fixation, pour over some OTC (or whatever cryo mounting media you 
prefer)and freeze it in n-hexane placed in a cylinder in liquid nitrogen.
Kind regards Andrea

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