cracked brain blues

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From:Bruce A Rasmussen <brasmuss@osf1.gmu.edu>
To:HistoNet@Pathology.swmed.edu
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Date:Tue, 20 Jul 1999 11:43:34 -0400 (EDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII


Hi histoneters,

I have been getting major cracking when freezing rat brains lately.
History: perfusion with 4% parafomaldahyde, post-fixation 4%
parformaldahyde one hour, cryoprotection 20% sucrose in PB 18hrs. Brains
were coated lightly with Lipshaws M-1 then dropped into isopentane which
was cooled with dry ice.  Where did I go wrong?
Thanks in advance

Bruce



--------------------------------------
Bruce Rasmussen
Predoc Fellow in Experimental Neuropsychology
The Krasnow Institute for Advanced Study
George Mason University
Mail Stop 2A1
Fairfax, VA 22030-4444
Office:703-993-4358
Lab:703-993-4369
Home:703-765-4570
Fax:703-993-4325
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