clarification
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From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Fri, 23 Jul 1999 10:26:08 -0600 |
Content-Type: | |
I believe Kerry is using thicker sections to be glued to a slide, hence the
use of epoxy. Superglue works better and glass slides are not the best
choice for ground sections (grind and polish). Plastic slides are
safer, less danger of injury when glass breaks, and it has happened -
you can ask Linda Jenkins about her cuts, not bruises.
Sections will also stay on plastic better, if you lightly grind the
surface to increase surface are (600 grit) coverslip thin bone slab aka
section onto plastic slide. Thin slab sections tend to detach from glass
with superglue and will not stay down during grinding/polishing or come
off later, making viewing a nightmare.
For thin microtomed PMMA, some use Plus charge or use Haupts gelatin on
the slides.
Gayle Callis
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