anti-IL-6 antibody

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From:MARUSSIG Myriam <myriam.marussig@BIOVECTOR.com>
To:"'HistoNet@pathology.swmed.edu'" <HistoNet@Pathology.swmed.edu>
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Date:Thu, 22 Jul 1999 17:56:44 +0200
Content-Type:text/plain

		Marcia,
		I have used the clone MP5-20F3 to stain murine IL-6 in
frozen tissues.  It works pretty well on 2 % formaldehyde fixed tissu
permealized with 0,1 % of saponin. Saponin has to be present during the
staining. I used 2ug/ml overnight. To my knowledge you will not find a
clone to stain paraffins because cytokines are very sensitive and are no
more recognized after parafin processing.
		I hope this will help you. Good luck !

		PS : Please specify the species of the protein you want
to stain
		(murine, human...) we will gain time
		Thanks

		Myriam Marussig
		Manager, Biological Studies
		Biovector Therapeutics S. A.
		Labege, France



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