Trouble with PMMA sections adhering

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From:Kerry B Cook <kbc@U.Arizona.EDU>
To:Tom Wells <tomers@home.com>
Reply-To:
Date:Tue, 20 Jul 1999 10:37:20 -0700 (MST)
Content-Type:TEXT/PLAIN; charset=US-ASCII


	I am having great difficulty in getting my PMMA sections to remain
on my glass slide during staining with a modifed H&E protocol for plastic
sections. I am using a 5 minute epoxy and my sections are all less than 90
um. The sections are taking up the stain, but the epoxy is giving out
before I can even get them into Eosin.

	Please send any suggestions or protocols that may be out there.

	These samples are vascular stents that contain metal. 

	Thank you,
	
	Kerry Cook CVT, LMT
	University of Arizona
	Department of Surgery
	Section of Surgical Research




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