Re: patchy keratin signal

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From:"John C. Dennis" <>
To:Heike Grabsch <>
Date:Mon, 19 Jul 1999 11:20:02 -0500 (CDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII


We've been using Fisher brand "Superfrost Plus" throughout.  

Since I put my question out last week, I've run an assay on Bouin's fixed
frozen tissue with similar results.  I'm beginning to think that maybe
the antibody lot is from a bunny of a different stripe.

Please let me know how your latest test works out.

Yours sincerely mystified

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

On Sat, 17 Jul 1999, Heike Grabsch wrote:

> Hi John,
> did you use the same slide-type all the time?
> We are having actually a serious similar problem with patchy staining 
> with several antibodies, but not all. It seems to correlate with a change 
>  from Superfrost Plus slides to Slides called "Histobond". The tissue 
> sections stick to the slides as nicely as before, and they cost onyl half 
> the price. This is why we changed to this other brand. However, now, not 
> just looking for tissue adherance but also evaluating immunohistochemical 
> staining properties, we seem to have a difference with patchy stainign as 
> you described. Next week I'll perform more experiments to come to a final 
> conclusion comparing different tissues sections on both slide types in 
> parallel etc.
> Heike

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