Pat,

Check out this paper:  J Neurosci Meth 85, 129-139 (1998).  It specifically 
addresses these issues.

Karen Larison in Oregon 


Date:          Fri, 16 Jul 1999 12:22:47 -0700
From:          "Patrick M. Haley " <pmhales@cybergap.net>
Subject:       in-situ question
To:            "Histonet" <HistoNet@Pathology.swmed.edu>

I am posting this for a friend running in-situ via a kit method.
They are wondering how critical the RNAse precautions are during sectioning?
Taking an educated guess, I told her very critical, (unless you want to
destroy what you are trying to demonstrate). This person may be trying to
take some short-cuts.
Any thoughts or experiences about this subject?

Many thanks!!

Pat Haley
HTN, inc.
www.histology.net