Re: formol mercuric chloride
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From: | "Bryan Llewellyn" <bryand@netbistro.com> |
To: | "'Histonet'" <histonet@Pathology.swmed.edu>, "Rena Krol" <Rena.Krol@usm.edu> |
Reply-To: | |
Date: | Mon, 26 Jul 1999 17:22:33 -0700 |
Content-Type: | text/plain; charset="iso-8859-1" |
This is otherwise known as formal sublimate.
Saturated aqueous mercuric chloride 9 parts
Concentrated formalin (40%) 1 part.
Keeps for years.
Some add a very small amount of acid fuchsin to colour it red as a warning.
It is extremely poisonous.
Fixation time is about 2 hours to overnight. Remove mercury pigment. Avoid
all metals (forceps, cassette lids etc).
For years I recycled mine. Simply pour the used fixative into a container.
Filter and re-use. I never noticed any deterioration in fixation at all.
It certainly beats going to all that trouble to dispose of mercuric chloride
as a hazardous waste.
Bryan Llewellyn
----- Original Message -----
From: Rena Krol <Rena.Krol@usm.edu>
To: <Histonet@Pathology.swmed.edu>
Sent: July 26, 1999 9:46 AM
Subject: formol mercuric chloride
> Hi Histonetters,
>
> One of the techs in our lab is looking for a recipe for formaol mercuric
> chloride. We can't find it in any of our texts, either old or new. Can
> anyone help? Thanks!
> Rena Krol
>
>
>
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