Hi Maureen,
I have had difficult tissues in the past and used Sta-On from Surgipath
#03105, in deionized water using plain slides.  I drained sections in
vertical position for 20 minutes and placed on a warming stage at 60
overnight, and they survived the HIER.  I also use this for brain sections.
Shirley Powell

Maureen Decorah wrote:

>  I'm looking for the ultimate slide adhesive. I need to do antigen
> retrevial with high heat and my connective tissue, the sclera, doesn't
> adhere well. I have used Vectabond and Plus slides but, alas, the same
> still happens.
>
>         ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>         Maureen Decorah, Research Animal Resources Center
>         385A Enzyme Institute, 1710 University Avenue
>         University of Wisconsin - Madison, Madison, WI 53705-4098
>         (608) 262-0933  Fax(608) 265-2698  E-mail: decorah@rarc.wisc.edu
>         ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~