Dana- Was the Oil red O requested on a parathyroid frozen? Intracellular
lipid droplets indicate a functionally suppressed "normal"  parathyroid
while adenomas and chief cell hyperplasias lack intracellular lipid. 
Jeff Silverman

----------
> From: Dana Settembre <settembr@UMDNJ.EDU>
> To: Hagerty, Marjorie A. <mhagerty@emc.org>
> Cc: 'Histonet' <histonet@Pathology.swmed.edu>
> Subject: Re: Rapid ORO
> Date: Monday, July 19, 1999 8:06 AM
> 
> On Fri, 16 Jul 1999, Hagerty, Marjorie A. wrote:
> 
> > Quite a surprising thing happened today at work before I arrived. After
> > cutting frozens on a case, the pathologist requested an Oil Red O. No
> > problem. However, it turned out that he wanted it stat before he called
the
> > OR. He's been here for about a year but this was a new one on us
because it
> > had never happened before and wasn't part of our protocol. 
> > 
> > Does anyone have a "rapid" procedure for ORO? Ours takes awhile.
> > 
> > Thanks in advance for any help you can give me.
> > 
> > Marg
> > EMC, Rancho Mirage, CA
> > 
> Marg
> I copied a "Rapid Oil Red O" stain from off the Histonet two years ago.
> It was put on by Gayle Callis.  She referenced Chuck Churkian, Rochester
> NY and Lillie and Fullmer, H Histopathological Technic and Practicl
> Histochemistdry, 4th Ed, 1976,  p 566.
> 
> Frozen sections fixed with buff formalin
> 
> Stain  in 0.5g Oil redO in dextrin 20 min
> rinse briefly with running tap
> counterstain with hematox of choice, blue, and coverslip with aqueous
> mounting media.
> 
> Reagents:  Dissolve 0.5 g oil read O in abs isopropyl alc, stir
overnight.
> Dissolve 1 g dextrin (Sigma type III, or bacteriological grade dextrin)
in
> 100ml distilled water.  Working solution is 60 mls stock oil red O and 40
> mls 1% dextrin, let sol'n stand for a day or more before using.
> 
> filter before using, and sol'n is stable for several months.  It has been
> reported from C. Churkian that it works on paraffin sections, provided
> some lipid is left after processing.  
> 
> Copied from Gayle Callis Fri,  July 18, 1997 off the Histonet.
> 
> Dana Settembre
> Immunohistochemistry Lab
> University Hospital
> Newark, NJ
>