Re: Rapid ORO

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From:Dana Settembre <settembr@UMDNJ.EDU>
To:"Hagerty, Marjorie A." <>
Date:Mon, 19 Jul 1999 08:06:19 -0400 (EDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Fri, 16 Jul 1999, Hagerty, Marjorie A. wrote:

> Quite a surprising thing happened today at work before I arrived. After
> cutting frozens on a case, the pathologist requested an Oil Red O. No
> problem. However, it turned out that he wanted it stat before he called the
> OR. He's been here for about a year but this was a new one on us because it
> had never happened before and wasn't part of our protocol. 
> Does anyone have a "rapid" procedure for ORO? Ours takes awhile.
> Thanks in advance for any help you can give me.
> Marg
> EMC, Rancho Mirage, CA
I copied a "Rapid Oil Red O" stain from off the Histonet two years ago.
It was put on by Gayle Callis.  She referenced Chuck Churkian, Rochester
NY and Lillie and Fullmer, H Histopathological Technic and Practicl
Histochemistdry, 4th Ed, 1976,  p 566.

Frozen sections fixed with buff formalin

Stain  in 0.5g Oil redO in dextrin 20 min
rinse briefly with running tap
counterstain with hematox of choice, blue, and coverslip with aqueous
mounting media.

Reagents:  Dissolve 0.5 g oil read O in abs isopropyl alc, stir overnight.
Dissolve 1 g dextrin (Sigma type III, or bacteriological grade dextrin) in
100ml distilled water.  Working solution is 60 mls stock oil red O and 40
mls 1% dextrin, let sol'n stand for a day or more before using.

filter before using, and sol'n is stable for several months.  It has been
reported from C. Churkian that it works on paraffin sections, provided
some lipid is left after processing.  

Copied from Gayle Callis Fri,  July 18, 1997 off the Histonet.

Dana Settembre
Immunohistochemistry Lab
University Hospital
Newark, NJ

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