Re: PAS stain

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From:"Alan Bright" <>
To:"Ronnie Houston" <>, <>
Date:Mon, 26 Jul 1999 12:37:53 +0100
Content-Type:text/plain; charset="iso-8859-1"

Hi Tim,

Ronnie makes a valid point here, we have been supplying cryostats for this
application for many years, and make a point to inform users that the
motorised cutting is an absolute necessity for achieving constant thickness
of sections.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margarets Way
PE18 6EB

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Web Site:

-----Original Message-----
From: Ronnie Houston <>
To: <>
Cc: <>
Date: Saturday, July 24, 1999 12:28
Subject: PAS stain

>With reference to your PAS density problems, are you cutting your sections
>using a motorized microtome/cryostat? If not, that could well be your
>problem. No matter the experience of the tech, it is impossible to cut
>sections at the same speed, and thus the same thickness. The only way to
>confidently cut sections of the same thickness is to use a motorized
>We came across the same problems many years ago in Scotland when we were
>quantifying enzyme activities in individual muscle fibres using
>There is a whole text that mentions many of the problems associated with
>quantitative histochemistry with reference to enzyme histochemistry, but
>there is much useful information for anyone wanting to quantitate in
>pathology. I suspect it is out-of-print now, but it may be in your nearest
>Med School library.
>"Trends in Enzyme Histochemistry and Cytochemistry" Ciba Foundation
>Symposium 73, Excerpta Medica 1980, ISBN 0 444 90135 3.
>Ronnie Houston
>Cytochemistry & Molecular Pathology
>Texas Scottish Rite Hospital for Children
>Get Free Email and Do More On The Web. Visit

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