Re: PAS stain

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From:"rudy gutierrez" <>
To:"HistoNet Server" <>
Date:Thu, 22 Jul 1999 10:15:41 -0700
Content-Type:text/plain; charset="iso-8859-1"

Dear Tim,
                   Sounds like you might have thick and thin sections.

-----Original Message-----
From: Tim Fairchild <>
To: HistoNet Server <>
Date: Thursday, July 22, 1999 4:05 AM
Subject: PAS stain

>When we are cutting our muscle block and subsequently staining for PAS, I
>noticed that a difference in staining intensity exists, even on the same
>(i.e. same block, simply the next section).  Since we are using the
>intensity as an indicator of glycogen stores (via densitometry) it is
>to ensure standardisation across all samples measured.  There is no
>evident, it is just that some sections come out darker than the others.
>Any thoughts on this?
>Thanks in advance
>Timothy J. Fairchild B.Sc. (Hons)
>PhD Candidate
>Co-ordinator for Centre of Athletic Testing
>Department of Human Movement and Exercise Science
>Nedlands, Western Australia 6907
>Telephone: (+61 8) 9380 2793
>Facsimile:  (+61 8) 9380 1039

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