Dear Tim,
                   Sounds like you might have thick and thin sections.
Rudy
imebrudy@inreach.com

-----Original Message-----
From: Tim Fairchild <timf@cyllene.uwa.edu.au>
To: HistoNet Server <HistoNet@Pathology.swmed.edu>
Date: Thursday, July 22, 1999 4:05 AM
Subject: PAS stain


>When we are cutting our muscle block and subsequently staining for PAS, I
have
>noticed that a difference in staining intensity exists, even on the same
slide
>(i.e. same block, simply the next section).  Since we are using the
staining
>intensity as an indicator of glycogen stores (via densitometry) it is
important
>to ensure standardisation across all samples measured.  There is no
streaking
>evident, it is just that some sections come out darker than the others.
>
>Any thoughts on this?
>
>Thanks in advance
>Tim.
>-----------------------------------------------------------------
>Timothy J. Fairchild B.Sc. (Hons)
>PhD Candidate
>Co-ordinator for Centre of Athletic Testing
>Department of Human Movement and Exercise Science
>Nedlands, Western Australia 6907
>Telephone: (+61 8) 9380 2793
>Facsimile:  (+61 8) 9380 1039
>Email: timf@cyllene.uwa.edu.au
>http://www.general.uwa.edu.au/~hmweb/index.htm
>
>
>
>
>