Re: PAS stain

<< Previous Message | Next Message >>
From:rkline@emindustries.com
To:Tim Fairchild <timf@cyllene.uwa.edu.au>
Reply-To:
Date:Thu, 22 Jul 1999 09:22:38 -0400
Content-Type:text/plain; charset=us-ascii

Tim,

It sounds to me that you are getting thick and thin sections on the slides.

Try rough cutting the block and placing it on ice which has begun to melt
when the tissue begins to appear  opaque it  is an indication that the
tissue has been hydrated.  You may have to discard the first few sections.

Sectioning should be more even once the tissue is softened.  Muscle does
have the tendency to be brittle.

Rande




Tim Fairchild <timf@cyllene.uwa.edu.au> on 07/22/99 05:45:21 AM

To:   HistoNet Server <HistoNet@Pathology.swmed.edu>
cc:
Subject:  PAS stain




When we are cutting our muscle block and subsequently staining for PAS, I
have
noticed that a difference in staining intensity exists, even on the same
slide
(i.e. same block, simply the next section).  Since we are using the
staining
intensity as an indicator of glycogen stores (via densitometry) it is
important
to ensure standardisation across all samples measured.  There is no
streaking
evident, it is just that some sections come out darker than the others.

Any thoughts on this?

Thanks in advance
Tim.
-----------------------------------------------------------------
Timothy J. Fairchild B.Sc. (Hons)
PhD Candidate
Co-ordinator for Centre of Athletic Testing
Department of Human Movement and Exercise Science
Nedlands, Western Australia 6907
Telephone: (+61 8) 9380 2793
Facsimile:  (+61 8) 9380 1039
Email: timf@cyllene.uwa.edu.au
http://www.general.uwa.edu.au/~hmweb/index.htm











<< Previous Message | Next Message >>