Re: Endogenous Biotin in Mast Cells
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From: | "Tony Henwood" <henwood@mail.one.net.au> |
To: | "'histonet@Pathology.swmed.edu'" <histonet@Pathology.swmed.edu>, "Donovan, Mark" <M.Donovan@alfred.org.au> |
Reply-To: | |
Date: | Sun, 25 Jul 1999 21:41:48 +0000 |
Content-Type: | text/plain; charset=US-ASCII |
Dear Mark,
> 1). Does anyone know if mast cells posses any endogenous biotin (a little or a lot?).
> 2). Does anyone know how to block endogenous biotin (apart from using a commercial kit).
Bussolati and Gugliotta described binding of ABC to mast
cells. They believe this to be due to both the binding of
avidin basic residues as well as peroxidase to the sulphate
groups of heparin. They showed that this can be prevented by
using the ABC solution at a pH of 9.4. This high pH does not
affect previous binding of localisation antibody nor the
affinity of biotin for avidin. They also showed that this
nonspecific binding can also be blocked by a 30 minute
pretreatment of sections with a synthetic basic polypeptide eg
poly L lysine (0.01% in PBS pH 7.6).
Bussolati & Gugliotta (1983) J.Histochem.Cytochem., 31(12):1419-1421.
I hope this helps,
Tony
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA
http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html
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