Re: Cyto keratin problem (ventana)

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From:"Jeff Silverman" <peptolab@hamptons.com>
To:<BEHLJACOBS@aol.com>, <histonet@pathology.swmed.edu>
Reply-To:
Date:Fri, 30 Jul 1999 19:21:29 -0400
Content-Type:text/plain; charset=ISO-8859-1

Actually, smooth muscle in uterus and fallopian tube often show reactivity
with cytokeratin AE1/AE3, this is well documented in the literature and I
have seen it in my own material. 
Jeff Silverman

----------
> From: BEHLJACOBS@aol.com
> To: histonet@pathology.swmed.edu
> Subject: Cyto keratin problem (ventana)
> Date: Friday, July 30, 1999 6:30 PM
> 
> One of our histotech's ran a cyto keratin on our Ventana today.  Our
problem 
> is that  the keratin stained nicely positive and the smooth muscle
stained 
> positive also.  We have never had this happen before.  One of the
pathologist 
> is concerned about carryover.  In the same run was a muscle specific
actin.  
> He is worried that some of the muscle specific actin was rinsed on to the

> case being stained for keratin.  Any help would be appreciated.  I really

> don't have specifics because I am not at work and I didn't do the run.
> 
> Ellen Jacobson
> Marquette General Hospital
> Marquette, Michigan



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