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To:"Carson, Karla" <>
Date:Wed, 21 Jul 1999 21:52:34 -0400
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NSH just had a teleconference on this TODAY - Wed. July 21, 1999.

Contact the NSH office at 301-262-6221. Ask them for Jennifer's
phone number, the person who gave the teleconference today. I
forget her last name, and I'm at home, and that's at work.

You really need a good procedure. THIS IS NOTHING TO GOOF UP ON.

Basically, you will need to fix the tissue in 10% NBF for several
days, place it in 90% formic acid for an hour, go back into NBF,
hand process in plastic dishes, collect all the fixation and processing
solutions and dishes for special sterilization and incineration,
wear all the personal protective equipment you can, cover your
microtomes with plastic wrap or aluminum foil to collect all 
the shavings (to be sterilized and incinerated), hand stain 
in plastic disposable containers (and again collect all 
staining solutions to be sterilized and incinerated). 

I hope someone will be able to contact Jennifer early
tomorrow, or that someone will be able to email you their
procedure. (Sorry, I got a new computer at work, and literally
cannot do a thing with it. But that's another story.)

Also, maybe someone can email you the address of the
Histonet archives. I can't find my bookmark on it.
I know this has been discussed before, so you might be able
to find a procedure from previous postings.

Good luck.

Peggy A. Wenk, HTL(ASCP)
William Beaumont Hospital
Royal Oak, MI 48073

"Carson, Karla" wrote:
> We are doing a brain biopsy tomorrow morning... just now got the word  for
> possible Creutzfeldt-Jacob Disease.  How are you handling the
> fixation/processing of surgical cases involving CJD?
> Karla Carson HT/HTL(ASCP)
> Regional Pathology Supervisor
> Mercy Healthcare Sacramento
> 916-453-4494
> <>

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