Re: CD 30

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To:Katri Tuomala <>, Donna Sitrin <>
Date:Wed, 21 Jul 1999 09:33:38 -0500
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We've had better results since switching to EDTA and using the steamer 20
min with 20 min cool down.  I also use a 30 minute primary incubation and
1:50 with Dako LSAB2 detection.
Bonnie Whitaker

At 10:38 PM 7/21/99 -0400, Katri Tuomala wrote:
>Dear Donna,
>We use Dako's CD30, clone BerH2, at a dilution of 1:40 and incubate at
>room temperature overnight. We use HIER in citrate buffer pH 6.0 for 15
>min., let cool for 20 min. This protocol works well for us. We used to
>incubate the primary for 1 hour and ended up with some false negatives.
>Our control is Hodgkins with Reed-Sternberg cells.
>Hope this helps.
>Katri Tuomala
>Anatomic Pathology
>St.Joseph's Hospital
>Hamilton, Ontario, Canada

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