Dear Donna,
We use Dako's CD30, clone BerH2, at a dilution of 1:40 and incubate at
room temperature overnight. We use HIER in citrate buffer pH 6.0 for 15
min., let cool for 20 min. This protocol works well for us. We used to
incubate the primary for 1 hour and ended up with some false negatives.
Our control is Hodgkins with Reed-Sternberg cells.
Hope this helps.
Katri

Katri Tuomala
Anatomic Pathology
St.Joseph's Hospital
Hamilton, Ontario, Canada