Re: BrdU and acridine orange
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From: | "Tony Henwood" <henwood@mail.one.net.au> |
To: | histonet@Pathology.swmed.edu, Deb Boswell Lane <dbl@aretha.jax.org> |
Reply-To: | |
Date: | Wed, 28 Jul 1999 21:04:42 +0000 |
Content-Type: | text/plain; charset=US-ASCII |
Dear Deb,
> Has anyone heard of a staining procedure for tissue to determine single
> stranded vs. double stranded DNA using acridine orange? We're helping a
> lab trouble shoot their BrdU procedure, and this procedure was mentioned.
> The single stranded DNA will fluoresce a different color than the double
> stranded DNA when stained with acridine orange in this procedure. Thanks
> for any information!
I have used acridine orange to differentiate DNA and RNA many years
ago and the only reference I can get my hands on is:
Maruno, M., Yanagihara, T., (1990) "Progressive loss of messenger
RNA and delayed neuronal death following transient cerebral
ischemia in gerbils" Neuroscience Letters 115:155-160.
I hope this helps, Tony
.
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA
http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html
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