Rapid Oil Red O

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From:"Hagerty, Marjorie A." <mhagerty@emc.org>
To:"'Histonet'" <histonet@Pathology.swmed.edu>
Date:Tue, 20 Jul 1999 15:21:02 -0700
Content-Type:text/plain; charset="iso-8859-1"

Thank you to all who responded to my "rapid" Oil Red O question. 

It is interesting, but the regular Oil Red O that we do is made with alcohol
and the slide is only treated for 5-10 minutes as opposed to the 20 minutes
most of you have mentioned. The references are: Histopathologic Technic and
Practical Histochemistry, R.D. Lillie and Harold M. Fullmer and Ann  Preece.

What takes the most time is the fixation. We have been fixing the sections
with formaldehyde vapor for 15 minutes in a 60 C oven to prevent section

I think I will go ahead and try to fix in 10% formalin and see if we have
better luck than previously in keeping the sections on the slide. This could
result in the total time, start to finish, to be about 7 or 8 minutes.

I am hesitant to try the microwave because I would think excessive heat
might alter the lipids? If anyone does have any luck with this, I would be

EMC, Rancho Mirage, CA

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