Be sure to use Superfrost Plus slides. What type of probe are you using?
Oligos hybridize at lower temps than RNA probes and allow one to perform ISH
under "gentler" conditions. If you have to use protease digestion, the conc.
should be minimal.

Brett


Brett M. Connolly, Ph.D.
Senior Research Biologist
Dept. of Human Genetics
Merck Research Laboratories
WP26A-3000
PO Box 4
West Point, PA 19486
tel. 215-652-2501
FAX 215-652-2075


> ----------
> From: 	Hawkins, Hal[SMTP:hhawkins@SBI.UTMB.EDU]
> Sent: 	Monday, July 19, 1999 3:48 PM
> To: 	'histonet'
> Subject: 	in situ
> 
> 
> 
> We are starting a project in which we want to do in situ
> hybridization (for IL-8 mRNA) on cytospin preparations.
> So far, most of the cells are coming off.  Does anyone
> have a method that will allow us to keep the cells tightly
> adherent to the slide during the processes needed for
> hybridization?
> 
> Thanks
> 
> Hal Hawkins, UTMB Galveston  
>