RE: SENTINAL NODE PROTOCOL

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From:"Tapper, Sheila" <STapper@smdc.org>
To:"'Coni Forney'" <conif@MEDCENOA.WPAFB.AF.MIL>, histonet@Pathology.swmed.edu
Reply-To:
Date:Fri, 16 Jul 1999 11:04:11 -0500
Content-Type:text/plain; charset="iso-8859-1"

We have just implemented a sentinal node protocol here.  For each block, we
cut 5 sections.  1,3,and 5 are stained with H&E.  Sections 2 and 4 are
stained with cytokeratin.  We have used the protocol 3 times, and each time
it has been a disaster!!!!  Our pathologists don't seem to know what the
work "protocol" means!!!  They have amended the protocol each time.  I have
sent the original protocol back to the pathologists to refine.  But this is
our starting point.  I would be really interested to hear what others are
doing as well...perhaps I could offer some suggestions to our
pathologists!!!

Sheila Tapper
SMDC Health System
Duluth, MN
Stapper@smdc.org

	-----Original Message-----
	From:	Coni Forney [SMTP:conif@MEDCENOA.WPAFB.AF.MIL]
	Sent:	Friday, July 16, 1999 7:34 AM
	To:	histonet@Pathology.swmed.edu
	Subject:	SENTINAL NODE PROTOCOL

	Help - looking for protocol for cutting sentinal nodes - what is
everyone
	using as far as levels, etc. We are currently taking several levels
at the
	beginning and then every 10 cuts until the block is exhausted. Is
there a
	standard protocol or is it site specific? Please advise. Thanks,

	Constance M. Forney MT HT(ASCP)
	Technical Supervisor, Histopathology
	USAF Medical Center
	Wright Patterson AFB, OH 



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