Dear Jennifer,

	When I worked in a hospital lab we used gloves, lab coat, eye protection &
kept the lid as closed as possible.  To disinfect a open container of
formalin was placed inside the cryo & the power turned off for at least 24
hours (we had 2 cryo's) then a quick wipe out with a disinfection agent
followed by absolute ethnol.  We used the same cryo for all our routines &
immuno's.

	I've heard of some labs that have a dedicated pair of cryo's for TB, one
is disinfected while the other is used, & there is a separate cryo for
routine work.  Other places just have 1 old cryo for TB, & do their
disinfection at night or weekends when they don't expect a sample.

	Some cryo manufacturers & suppliers now supply cleaning agents
specifically for cryo's.

	Regards

	Rob W.

At 10:38 AM 7/22/99 -0700, you wrote:
>Other than gloves and a lab coat, what type of PPE is the standard for
>frozen sections?  Does anyone wear masks (respirators) for  while doing
>frozen sectionsTB?  What about for cleaning the cryostats?
>Jennifer MacDonald
>San Antonio Community Hospital

R. Wadley, B.App.Sc. M.L.S, Grad.Dip.Sc.MM
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) 	+61 (2) 9385 3517
Ph (AH)	+61 (2) 9555 1239
Fax 	+61 (2) 9385 1591
E-mail	r.wadley@unsw.edu.au
www	http://www.micro.unsw.edu.au/caf.html