PS:

Another key consideration is the optimal dilution at which you will be
running your primaries.  It will be more likely to be cost effective to
purchase an antibody that runs optimally at 1:1000 as a concentrate than
one that requires a 1:30 titre to achieve an optimal stain.

> ----------
> From: 	Goodwin, Diana[SMTP:DGoodwin@CHSNJ.org]
> Sent: 	Tuesday, July 20, 1999 3:49 PM
> To: 	Histonet; 'Patrick M. Haley '
> Subject: 	RE: Immunoperoxidase kits
> 
> Hi, Patrick.
> 
> I use both concentrated and pre-diluted primary antibodies, having
> determined which to use by the volume of requests for the particular
> antibody.  For example, it is more cost effective for me to use a
> concentrated cytokeratin because I get allot of requests for it; on
> the
> other hand, my neuron specific enolase (NSE) is a pre-dilute because
> if
> I buy a concentrate, it will inevitably expire long before I use it
> all.
> 
> 
> You need to evaluate the type of surgery your institution performs and
> what kind of volume you're likely to expect for each particular
> antibody.  Also, many of the more expensive ones (e.g., ER and PR) are
> now packaged in smaller quantities in concentrated form, affording
> another option in lower volume situations.
> 
> Good Luck!
> 
> 
> Diana Goodwin,  HT
> Trenton,  NJ
> 
> 
> 
> > ----------
> > From: 	Patrick M. Haley [SMTP:pmhales@cybergap.net]
> > Sent: 	Wednesday, July 14, 1999 11:09 AM
> > To: 	Histonet
> > Subject: 	Immunoperoxidase kits
> > 
> > Fellow HistoNetters,
> >     We are researching the idea of offering immunoperoxidase
> staining.
> > My
> > question is, what companies sell kits that would include primary
> > antibody,
> > detection system, chromogen, and control slide in one package? We
> are
> > on a
> > limited budget.
> > 
> > Thank you for all your help.
> > 
> > Gayle, I received your safranin O/Fast Green procedure, I hope to
> try
> > it
> > next week.  Many thanks .
> > 
> > Pat Haley
> > Chief Technologist
> > HistoTechNologies, inc.
> > www.histology.net
> > 
> > 
> > 
>