RE: Immunoperoxidase kits

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From:"Goodwin, Diana" <>
To:Histonet <>, "'Patrick M. Haley '" <>
Date:Tue, 20 Jul 1999 15:49:11 -0400

Hi, Patrick.

I use both concentrated and pre-diluted primary antibodies, having
determined which to use by the volume of requests for the particular
antibody.  For example, it is more cost effective for me to use a
concentrated cytokeratin because I get allot of requests for it; on the
other hand, my neuron specific enolase (NSE) is a pre-dilute because if
I buy a concentrate, it will inevitably expire long before I use it all.

You need to evaluate the type of surgery your institution performs and
what kind of volume you're likely to expect for each particular
antibody.  Also, many of the more expensive ones (e.g., ER and PR) are
now packaged in smaller quantities in concentrated form, affording
another option in lower volume situations.

Good Luck!

Diana Goodwin,  HT
Trenton,  NJ

> ----------
> From: 	Patrick M. Haley []
> Sent: 	Wednesday, July 14, 1999 11:09 AM
> To: 	Histonet
> Subject: 	Immunoperoxidase kits
> Fellow HistoNetters,
>     We are researching the idea of offering immunoperoxidase staining.
> My
> question is, what companies sell kits that would include primary
> antibody,
> detection system, chromogen, and control slide in one package? We are
> on a
> limited budget.
> Thank you for all your help.
> Gayle, I received your safranin O/Fast Green procedure, I hope to try
> it
> next week.  Many thanks .
> Pat Haley
> Chief Technologist
> HistoTechNologies, inc.

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