RE: IHC mounting media

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From:"Nocito, Joseph" <>
To:"''" <>, "Nocito, Joseph" <>
Date:Wed, 28 Jul 1999 07:01:32 -0500

I have no idea.  I was introduced to Crystalmount during my days with the
Code-On immuno stainer.  I don't know id Dr. Dave Briggoti had something to
say about that or not.  I didn't know you could coverslip over wet
Crystalmount until I started working here at Santa Rosa.  See, you learn
something new every time.

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas 

> -----Original Message-----
> From: []
> Sent:	Tuesday, July 27, 1999 6:54 PM
> To:	Nocito, Joseph
> Cc:
> Subject:	RE: IHC mounting media
> Joe, Katri, Doreen,
> I apply a coverslip over the Crystal/Mount after it has hardened to
> improve
> resolution.  Just dip the slide in xylene, drop on compatible mounting
> media and
> slap on a coverslip.  I've even used my Hacker robot coverslipper to do
> this.
> But I'm curious about using a coverslip directly over wet Crystal/Mount.
> This
> seems like such an easy answer, I wonder why the manufacturer doesn't
> suggest
> this?  Anyone have any ideas?
> Beth Roche
> Gore Hybrid Technologies
> Flagstaff, AZ

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