RE: IHC mounting media
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From: | "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org> |
To: | "'broche@wlgore.com'" <broche@wlgore.com>, "Nocito, Joseph" <joseph_nocito@srhc.iwhs.org> |
Reply-To: | |
Date: | Wed, 28 Jul 1999 07:01:32 -0500 |
Content-Type: | text/plain |
Beth,
I have no idea. I was introduced to Crystalmount during my days with the
Code-On immuno stainer. I don't know id Dr. Dave Briggoti had something to
say about that or not. I didn't know you could coverslip over wet
Crystalmount until I started working here at Santa Rosa. See, you learn
something new every time.
Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas
> -----Original Message-----
> From: broche@wlgore.com [SMTP:broche@wlgore.com]
> Sent: Tuesday, July 27, 1999 6:54 PM
> To: Nocito, Joseph
> Cc: Histonet@Pathology.swmed.edu
> Subject: RE: IHC mounting media
>
>
>
> Joe, Katri, Doreen,
> I apply a coverslip over the Crystal/Mount after it has hardened to
> improve
> resolution. Just dip the slide in xylene, drop on compatible mounting
> media and
> slap on a coverslip. I've even used my Hacker robot coverslipper to do
> this.
> But I'm curious about using a coverslip directly over wet Crystal/Mount.
> This
> seems like such an easy answer, I wonder why the manufacturer doesn't
> suggest
> this? Anyone have any ideas?
>
> Beth Roche
> Gore Hybrid Technologies
> Flagstaff, AZ
> broche@wlgore.com
>
>
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