RE: IHC mounting media

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From:"Nocito, Joseph" <joseph_nocito@srhc.iwhs.org>
To:"'P. Emry'" <emry@u.washington.edu>, Katri Tuomala <katri@istar.ca>
Reply-To:
Date:Tue, 27 Jul 1999 15:20:20 -0500
Content-Type:text/plain

Katri,
please be careful about using Crystalmount only.  My experience is that
after the Crystalmount hardens, it tends to develop an uneven surface.  This
is noticable whe nviewing the slides at 40x or higher.  It becomes hard to
focus up and down.  That is why I use a coverslip with the Crystalmount,
even though the manufacturer says not to (Sometimes I have to go against the
grain).  I have pulled 3-4 year old immuno slides that were stained with AEC
and to my suprise, the reaction was still there.  After everyone told me
that AEC fades, well, this just blew me away.  imagine, 4 year old slides
stained with AEC and STILL visible.  What a concept!!

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas 


> -----Original Message-----
> From:	P. Emry [SMTP:emry@u.washington.edu]
> Sent:	Tuesday, July 27, 1999 12:35 PM
> To:	Katri Tuomala
> Cc:	Rudman, Dorene M.; 'histonet@Pathology.swmed.edu'
> Subject:	Re: IHC mounting media
> 
> Hi Katri,
> 
> Am I reading this right?  You just cover the specimen and let it harden?
> Is there a special way to apply it?  Why haven't I heard of this...sounds
> terrific.
> Thanks,
> Trisha, U of WA, Seattle
> 
> On Tue, 27 Jul 1999, Katri Tuomala wrote:
> 
> > Hi Doreen,
> > There are several vendors that carry a permanent aqueous mounting media,
> > which preserves the staining well. My experience is with Crystalmount
> > (Biomeda?). It is expensive, but does not require the use of coverslips,
> > it dries hard. Hope this helps.
> > Katri 
> > 
> > Katri Tuomala
> > Anatomic Pathology
> > St.Joseph's Hospital
> > Hamilton, Ontario, Canada
> > 
> > 
> 
> Trisha  
> 



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